Lactoferricin B causes depolarization of the cytoplasmic membrane of Escherichia coli ATCC 25922 and fusion of negatively charged liposomes

FEBS Letters

Haukland, H.H¹  Vorland, L.H¹  Olsvik, ع  Ulvatne, H¹ 

[1] Department of Medical Microbiology, P.O. Box 56, University Hospital of Tromsø, N-9038 Tromsø, Norway

关键词: Antimicrobial peptide;    Lactoferricin;    Liposome;    Mode of action;    Lfcin B;    bovine lactoferricin;    DPPC;    diphosphatidyldipalmitoylcholine;    DPPG;    diphosphatidyldipalmitoylglycerol;    DPPS;    diphosphatidyldipalmitoylserine;    JC-1;    5;    5′;    6;    6′-tetrachloro-1;    1′;    3;    3′-tetraethyl-benzimidazolcarbocyanine iodide;    PI;    propidium iodide;    CFU;    colony forming units;    MIC;    minimal inhibitory concentration;    MBC;    minimal bactericidal concentration;    IUV;    intermediate unilamellar vesicles;    SUV;    small unilamellar vesicles;    BPW;    bacto peptone water;   

DOI  :  10.1016/S0014-5793(01)02233-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antimicrobial peptides have been extensively studied in order to elucidate their mode of action. Most of these peptides have been shown to exert a bactericidal effect on the cytoplasmic membrane of bacteria. Lactoferricin is an antimicrobial peptide with a net positive charge and an amphipatic structure. In this study we examine the effect of bovine lactoferricin (lactoferricin B; Lfcin B) on bacterial membranes. We show that Lfcin B neither lyses bacteria, nor causes a major leakage from liposomes. Lfcin B depolarizes the membrane of susceptible bacteria, and induces fusion of negatively charged liposomes. Hence, Lfcin B may have additional targets responsible for the antibacterial effect.

【 授权许可】

Unknown