Activity and mRNA abundance of Δ‐5 and Δ‐6 fatty acid desaturases in two human cell lines

FEBS Letters

Ponton, André¹  Allen, Stephen J.¹  de Antueno, Roberto J.¹  Winther, Michael D.¹ 

[1]QuantaNova Canada Ltd., P.O. Box 818, Kentville, N.S., Canada B4N 4H8

关键词: Desaturase;    Elongation;    Gene expression;    Polyunsaturated fatty acid;    Chang;    ZR-75-1;    Essential fatty acid metabolism;    s.a.;    specific activity;    PUFA;    polyunsaturated fatty acid;    fatty acids are designated with a number that refers to the number of carbons in the chain;    the number after the colon indicates the number of double bonds and the n refers to the location of the nearest double bond from the methyl end;   

DOI  :  10.1016/S0014-5793(01)02209-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We analyzed fatty acid biosynthesis in Chang and ZR-75-1 cells. Both cell lines could desaturate and further elongate substrates for Δ-5 desaturase. ZR-75-1 but not Chang cells showed Δ-6 desaturation of 18:2n-6, 18:3n-3, 24:4n-6 and 24:5n-3. In both cell lines, the mRNA abundance can be related to Δ-5 or Δ-6 fatty acid desaturase activities. These results suggest that desaturase genes could have, at least in part, independent control mechanisms and that Δ-6 desaturase impairment is not specific to any particular step of the fatty acid metabolic pathways, which may diminish the rationale for the existence of at least two distinct enzymes.

【 授权许可】

Unknown