Identification of Primula fatty acid Δ6‐desaturases with n‐3 substrate preferences 1

FEBS Letters

Shewry, Peter R.¹  Michaelson, Louise V.¹  Beaudoin, Frédéric¹  Napier, Johnathan A.¹  Sayanova, Olga V.¹ 

[1] Long Ashton Research Station, Long Ashton, Bristol BS41 9AF, UK

关键词: Cytochrome b 5 fusion;    Desaturase;    γ-Linolenic acid;    Polyunsaturated fatty acid;    ALA;    α-linolenic acid;    EFA;    essential fatty acid;    GLA;    γ-linolenic acid;    LA;    linoleic acid;    LCB;    long chain base;    ORF;    open reading frame;    OTA;    octadecatetraenoic acid;    PUFA;    polyunsaturated fatty acid;   

DOI  :  10.1016/S0014-5793(03)00358-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fatty acid Δ⁶-desaturation, the first committed step in C₂₀ polyunsaturated fatty acid biosynthesis, is generally considered not to discriminate between n-3 and n-6 substrates. We previously identified higher plant species that showed preferential Δ⁶-desaturation of n-3 C₁₈ fatty acid substrates. A polymerase chain reaction-based approach was used to isolate ‘front-end’ cytochrome b ₅ fusion desaturases from Primula vialii Franchet and Primula farinosa L. Functional analysis in Saccharomyces cerevisiae identified fatty acid Δ⁶-desaturases with a strong specificity for the n-3 substrate α-linolenic acid (18:3Δ^9,12,15). These results indicate that the accumulation of octadecatetraenoic acid (18:4Δ^6,9,12,15) in planta is due to the activity of a novel n-3-specific fatty acid Δ⁶-desaturase.

【 授权许可】

Unknown