Functional analysis of the omega-6 fatty acid desaturase (CaFAD2) gene family of the oil seed crop Crambe abyssinica

BMC Plant Biology

Research Article

Bangquan Huang¹  Jens Gruber²  Li-Hua Zhu³  Eibertus N van Loo⁴  Elma MJ Salentijn⁴  Frans A Krens⁴  Richard GF Visser⁴  Annemarie C Dechesne⁴  Jihua Cheng⁵  Weicong Qi⁵ 

[1]College of Life Science, Hubei University, Wuhan, People’s Republic of China

[2]Institute for Biology I-Botany, RWTH Aachen University, Aachen, Germany

[3]Plant Breeding and Biotechnology, Swedish University of Agricultural Science, Alnarp, Sweden

[4]Wageningen UR Plant Breeding, P.O. Box 16, 6700, Wageningen, AA, The Netherlands

[5]Wageningen UR Plant Breeding, P.O. Box 16, 6700, Wageningen, AA, The Netherlands

[6]College of Life Science, Hubei University, Wuhan, People’s Republic of China

关键词: Crambe abyssinica;    Fatty acid desaturase 2;    Oil crop;    Oleic acid;    Polyunsaturated fatty acid;    RNAi;    Gene expression;   

DOI  :  10.1186/1471-2229-13-146

 received in 2013-05-08, accepted in 2013-09-24,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundCrambe abyssinica produces high erucic acid (C22:1, 55-60%) in the seed oil, which can be further increased by reduction of polyunsaturated fatty acid (PUFA) levels. The omega-6 fatty acid desaturase enzyme (FAD2) is known to be involved in PUFA biosynthesis. In crambe, three CaFAD2 genes, CaFAD2-C1, CaFAD2-C2 and CaFAD2-C3 are expressed.ResultsThe individual effect of each CaFAD2 gene on oil composition was investigated through studying transgenic lines (CaFAD2-RNAi) for differential expression levels in relation to the composition of seed-oil. Six first generation transgenic plants (T1) showed C18:1 increase (by 6% to 10.5%) and PUFA reduction (by 8.6% to 10.2%). The silencing effect in these T1-plants ranged from the moderate silencing (40% to 50% reduction) of all three CaFAD2 genes to strong silencing (95% reduction) of CaFAD2-C3 alone. The progeny of two T1-plants (WG4-4 and WG19-6) was further analysed. Four or five transgene insertions are characterized in the progeny (T2) of WG19-6 in contrast to a single insertion in the T2 progeny of WG4-4. For the individual T2-plants of both families (WG19-6 and WG4-4), seed-specific silencing of CaFAD2-C1 and CaFAD2-C2 was observed in several individual T2-plants but, on average in both families, the level of silencing of these genes was not significant. A significant reduction in expression level (P < 0.01) in both families was only observed for CaFAD2-C3 together with significantly different C18:1 and PUFA levels in oil.ConclusionsCaFAD2-C3 expression is highly correlated to levels of C18:1 (r = -0.78) and PUFA (r = 0.75), which suggests that CaFAD2-C3 is the most important one for changing the oil composition of crambe.

【 授权许可】

Unknown   
© Cheng et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.