Identification of gcpE as a novel gene of the 2‐C‐methyl‐D‐erythritol 4‐phosphate pathway for isoprenoid biosynthesis in Escherichia coli

FEBS Letters

Campos, Narciso²  Rohmer, Michel¹  Boronat, Albert²  Rodrı́guez-Concepción, Manuel²  Seemann, Myriam¹ 

[1] Université Louis Pasteur/CNRS, Institut Le Bel, 4 rue Blaise, 67070 Strasbourg Cedex, France;Department de Bioquı́mica i Biologia Molecular, Facultat de Quı́mica, Universitat de Barcelona, C/Martı́ i Franquès 1, 08028 Barcelona, Spain

关键词: Isoprenoid biosynthesis;    2-C-methyl-D-erythritol 4-phosphate pathway;    GcpE;    Mevalonic acid;    CAT;    chloramphenicol acetyl transferase;    CDP-ME;    4-diphosphocytidyl-2-C-methyl-D-erythritol;    CDP-ME2P;    4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate;    DMAPP;    dimethylallyl diphosphate;    DXP;    1-deoxy-D-xylulose 5-phosphate;    DXR;    1-deoxy-D-xylulose 5-phosphate reductoisomerase;    DXS;    1-deoxy-D-xylulose 5-phosphate synthase;    IPP;    isopentenyl diphosphate;    ME-2;    4cPP;    2-C-methyl-D-erythritol 2;    4-cyclodiphosphate;    MEP;    2-C-methyl-D-erythritol 4-phosphate;    MVA;    mevalonate;    ORF;    open reading frame;   

DOI  :  10.1016/S0014-5793(00)02420-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis is essential in most eubacteria and plants and has remarkable biotechnological interest. However, only the first steps of this pathway have been determined. Using bioinformatic and genetic approaches, we have identified gcpE as a novel gene of the MEP pathway. The distribution of this gene in bacteria and plants strictly parallels that of the gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase, which catalyses the first committed step of the MEP pathway. Our data demonstrate that the gcpE gene is essential for the MEP pathway in Escherichia coli and indicate that this gene is required for the trunk line of the isoprenoid biosynthetic route.

【 授权许可】

Unknown