FEBS Letters | |
Protein prenylation in Schizosaccharomyces pombe | |
Magee, Anthony I.1  Armstrong, John2  Giannakouros, Thomas1  | |
[1] Laboratory of Eukaryotic Molecular Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK;Membrane Molecular Biology Laboratory, Imperial Cancer Research Fund, P.O. Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK | |
关键词: S. pombe; S. cerevisiae; Mevalonic acid; Prenylated protein; HMG-CoA; hydroxymethylglutaryl coenzyme A; MVA; mevalonic acid; SDS; sodium dodecyl sulphate; TCA; trichloroacetic acid; | |
DOI : 10.1016/0014-5793(92)80337-G | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
S. pombe is shown to be a powerful system for studies concerning attachment or polyisoprenoid moieties to proteins, due to its ability to take up exogenous mevalonic acid efficiently. The fission yeast can take up about 5% of the exogenously added mevalonic acid and incorporate ∼10% of this into protein. By contrast, the uptake obtained with the budding yeast S. cerevisiae is less than 0.5% HPLC analysis of total S. pombe protein-bound isoprenoids revealed that ∼55% of the counts co-migrated with the geranylgeraniol standard, while ∼45 of the counts co-migrated with farnesol. We could not detect any effects of mevinolin or other HMG-CoA reductase inhibitors in S. pombe.
【 授权许可】
Unknown
【 预 览 】
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