The formation of chlorophyll from chlorophyllide in leaves containing proplastids is a four‐step process

FEBS Letters

Schoefs, Benoît¹  Bertrand, Martine² 

[1]Laboratory of Biomembranes, University of South Bohemia at Ceske Budejovice, Branisovska 31, CZ-370 05 Ceske Budejovice, Czech Republic

[2]Institut National des Sciences et Techniques de la Mer, Conservatoire National des Arts et Métiers, Digue de Collignon, F-50110 Tourlaville, France

关键词: Pigment biosynthesis;    HPLC;    Greening;    Chlorophyll;    Esterification;    Last step of the chlorophyll biosynthetic pathway;    Chl;    chlorophyll a;    Chlide;    chlorophyllide a;    Pchlide;    protochlorophyllide a;   

DOI  :  10.1016/S0014-5793(00)02309-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The time course of the different esters of chlorophyllide (Chlide) during the formation of chlorophyll a (Chl) in embryonic bean leaves containing proplastids was investigated by HPLC. After the reduction of photoactive Pchlide (Pchlide) to Chlide, three intermediates, i.e. Chlide geranylgeraniol, Chlide dihydrogeranylgeraniol and Chlide tetrahydrogeranylgeraniol were detected before the formation of Chlide phytol, i.e. authentic Chl. The transformation of Chlide to Chl was found to be much faster in leaves containing proplastids than in etiolated leaves with etioplasts.

【 授权许可】

Unknown