HPLC determination of photosynthetic pigments during greening of etiolated barley leaves

FEBS Letters

Nakamura, Akimasa¹  Watanabe, Tadashi¹ 

[1]Institute of Industrial Science, University of Tokyo, Roppongi, Minato-ku, Tokyo 106-8558, Japan

关键词: Photosystem I;    Pigment biosynthesis;    Chlorophyll a′;    High performance liquid chromatography;    Greening;    Chl;    chlorophyll;    Pchlide;    protochlorophyllide;    Chlide;    chlorophyllide;    LHC;    light-harvesting complex;    PS;    photosystem;    RC;    reaction center;    GG;    geranylgeranyl;    DHGG;    dihydro-GG;    THGG;    tetrahydro-GG;    P;    phytyl;   

DOI  :  10.1016/S0014-5793(98)00344-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The temporal evolution of pigment composition during greening of etiolated barley leaves was investigated by reversed-phase HPLC with particular attention to chlorophyll (Chl) a′ (C13² epimer of Chl a), which had been detected by ourselves in photosystem (PS) I particles. At early stages of greening, the Chl a′/Chl a molar ratio rapidly increased to a level more than twice that in mature leaves, then gradually leveled off, accompanied by a growth of the Chl b/Chl a ratio, to the mature level. After 3 h of illumination, the temporal evolution of the Chl a′/Chl a molar ratio nearly paralleled that of the P700/Chl a ratio with a stoichiometry Chl a′/P700≅2: this strongly suggests that Chl a′ is biosynthesized as a constituent of the PS I reaction center complex.

【 授权许可】

Unknown