The bacteriochlorophyll biosynthesis gene, bchM, of Rhodobacter sphaeroides encodes S‐adenosyl‐L‐methionine: Mg protoporphyrin IX methyltransferase

FEBS Letters

Hunter, C.Neil¹  Gibson, Lucien C.D.¹ 

[1] Robert Hill Institute for Photosynthesis and Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2UH, UK

关键词: Photosynthesis;    Bacteriochlorophyll;    Chlorophyll;    Rhodobacter sphaeroides;    Methyltransferase;    R.;    Rhodobacter;    Bchl;    bacteriochlorophyll;    SAM;    S-adenosyl-l-methionine;    SDS-PAGE;    sodium dodecyl sulphate polyacrylamide gel electrophoresis;    HPLC;    high-pressure liquid chromatography;    IPTG;    isopropyl-βd-thiogalactosidase;    ORF;    open reading frame;   

DOI  :  10.1016/0014-5793(94)00934-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The bchM gene of Rhodobacter sphaeroides has been sequenced and then overexpressed in E. coli producing a protein of M _r approximately 27,500. Cell-free extracts of the transformed E. coli strain are able to methylate added Mg protoporphyrin, resulting in the formation of Mg protoporphyrin monomethyl ester. The identity of this product was verified by HPLC. The bchM gene product is therefore assigned to the methyltransferase step in bacteriochlorophyll biosynthesis.

【 授权许可】

Unknown