The cytotoxic lipid peroxidation product, 4‐hydroxy‐2‐nonenal, specifically inhibits decarboxylating dehydrogenases in the matrix of plant mitochondria

FEBS Letters

Millar, A.Harvey²  Leaver, Christopher J¹ 

[1] Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK;Plant Sciences Group and Department of Biochemistry, The University of Western Australia, Nedlands, W.A. 6907, Australia

关键词: 4-Hydroxy-2-nonenal;    Lipid peroxidation;    Pyruvate;    2-Oxoglutarate;   

DOI  :  10.1016/S0014-5793(00)01976-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

4-Hydroxy-2-nonenal (HNE), a cytotoxic product of lipid peroxidation, inhibits O₂ consumption by potato tuber mitochondria. 2-Oxoglutarate dehydrogenase (OGDC), pyruvate dehydrogenase complex (PDC) (both 80% inhibited) and NAD-malic enzyme (50% inhibited) are its major targets. Mitochondrial proteins identified by reaction with antibodies raised to lipoic acid lost this antigenicity following HNE treatment. These proteins were identified as acetyltransferases of PDC (78 kDa and 55 kDa), succinyltransferases of OGDC (50 kDa and 48 kDa) and glycine decarboxylase H protein (17 kDa). The significance of the effect of these inhibitions on the impact of lipid peroxidation and plant respiratory functions is discussed.

【 授权许可】

Unknown