Autocatalytic modification of human carbonyl reductase by 2‐oxocarboxylic acids

FEBS Letters

Ernst, Elsbeth¹  Bohren, Kurt M.¹  Wermuth, Bendicht¹ 

[1] Chemisches Zentrallabor der Universitätskliniken, Inselspital, CH-3010 Bern, Switzerland

关键词: Carbonyl reductase;    Enzyme modification;    Pyruvate;    2-Oxoglutarate;    Human;   

DOI  :  10.1016/0014-5793(93)80719-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Carbonyl reductase occurs in multiple molecular forms. Sequence analysis has yielded a carboxyethyllysine residue in one of the enzyme forms, suggesting that pyruvate has been incorporated in a posttranslational enzymatic reaction [Krook, M., Ghosh, D., Strömberg, R., Carlquist, M. and Jörnvall, H. (1993) Proc. Natl. Acad. Sci. USA 90,502-506]. Using highly purified carbonyl reductase from human brain we show that pyruvate and other 2-oxocarboxylic acids are bound to the enzyme in an autocatalytic reaction. The resulting enzyme forms were indistinguishable from the native enzyme forms by electrophoresis and isoelectric focusing.

【 授权许可】

Unknown