Gene expression profiling: monitoring transcription and translation products using DNA microarrays and proteomics

FEBS Letters

Østergaard, Morten²  Gromova, Irina²  Gromov, Pavel²  Pálsdóttir, Hildur²  Thykjaer, Thomas¹  Ørntoft, Torben F¹  Frederiksen, Casper¹  Kruhøffer, Mogens¹  Magnusson, Nils²  Celis, Julio E²  Yu, Jinsheng¹ 

[1] Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital, Skejby, DK-8200 Aarhus N, Denmark;Department of Medical Biochemistry and Danish Centre for Human Genome Research, University of Aarhus, Ole Worms Alle, Bldg. 170, DK-8000 Aarhus C, Denmark

关键词: Gene expression profile;    Microarray;    Proteome;    Two-dimensional gel electrophoresis;    Immunohistochemistry;    Bladder cancer;    2D PAGE;    two-dimensional polyacrylamide gel electrophoresis;    IEF;    isoelectric focusing;    IPG;    immobilized pH gradient;    NEPHGE;    non-equilibrium pH gradient electrophoresis;   

DOI  :  10.1016/S0014-5793(00)01771-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Novel and powerful technologies such as DNA microarrays and proteomics have made possible the analysis of the expression levels of multiple genes simultaneously both in health and disease. In combination, these technologies promise to revolutionize biology, in particular in the area of molecular medicine as they are expected to reveal gene regulation events involved in disease progression as well as to pinpoint potential targets for drug discovery and diagnostics. Here, we review the current status of these technologies and highlight some studies in which they have been applied in concert to the analysis of biopsy specimens.

【 授权许可】

Unknown