Effects of (-)-6,6'-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses

Revista Brasileira de Farmacognosia

Filho, Ademar A.S.¹  Lima, Thais C.¹  Universidade de Franca, Franca, Brazil¹  Universidade de São Paulo, Ribeirão Preto, Brazil¹  Bastos, Jairo K.¹  Silva, Márcio L.A.¹  Rodrigues, Vanderlei¹  Magalhães, Lizandra G.¹  Universidade do Estado de São Paulo, Ilha Solteira, Brazil¹  Cunha, Wilson R.¹  Gardinassi, Luiz G.¹  Yatsuda, Ana P.¹  Universidade Federal de Uberlândia, Patos de Minas, Brazil¹  Universidade de Juiz de Fora, Juiz de Fora, Brazil¹  Paula, Renato G. de¹  Morais, Enyara R.¹  Laurentiz, Rosangela S.¹  Garcia, Gustavo R.¹  Aguiar, Daniela P.¹ 

关键词:  (-)-6;    6'-dinitrohinokinin;    Lignan;    Mass spectrometry;    Proteome;    Schistosoma mansoni;    Two-dimensional gel electrophoresis;   

DOI  :  10.1016/j.bjp.2016.02.001

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (-)-6,6'-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (-)-6,6'-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies.

【 授权许可】

Unknown