Functional analysis of soybean dirigent genes

Dirigents (DIR);Lignin;Lignan;Soybean;Pathogen-induced defense

Berruezo, Florencia ; Domier, Leslie, ; ,Lambert, Kris ; Clough ; Steven J.

关键词: Dirigents (DIR);    Lignin;    Lignan;    Soybean;    Pathogen-induced defense;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/98165/BERRUEZO-THESIS-2017.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

In this project, the expression of the soybean dirigent genes and their effect on plant defense to pathogens was studied. Dirigent proteins, present in all of the major land plants, have been hypothesized to work in concert with oxidative enzymes to direct the synthesis of stereospecific lignans, such as (+) or (-)-pinoresinol, and lignins, from the selective coupling of specific stereoisomers of deprotonated alcohol molecules. Lignans and lignins have been shown to have antimicrobial properties and human health benefits when polymerized. The expression patterns in leaves of several genes encoding dirigent-like proteins in soybean suggest that these genes are pathogen-induced. The first goal of this research project was to identify all dirigent-like genes in soybean. Once identified, alignments and a phylogenetic tree of their protein sequences were made, and 3 selected ones were chemically synthesized and overexpressed in Arabidopsis thaliana to determine in the future if they function in these plants to enhance resistance to pathogens. In addition, I analyzed the promoter of the 3 selected dirigent genes that were determined to be the most pathogen-specific in its expression pattern. Transient transformation and disease assays were performed in Nicotiana benthamiana and soybean, showing possible reduction in the size of lesions induced by Sclerotinia sclerotiorum when the soybean dirigent-like genes were transiently expressed in N. benthamiana.