Changes in intramitochondrial cardiolipin distribution in apoptosis‐resistant HCW‐2 cells, derived from the human promyelocytic leukemia HL‐60

FEBS Letters

Pedrazzi, Jessica³  Salvioli, Stefano²  Troiano, Leonarda³  Castilla-Cortazar, Inma¹  Cossarizza, Andrea³  Garcia Fernandez, Maria¹  Moretti, Laura³ 

[1] Department of Human Physiology, University of Malaga, Campus Teatinos, 29080 Malaga, Spain;Department of Experimental Pathology, University of Bologna, via S. Giacomo 14, 40100 Bologna, Italy;Department of Biomedical Sciences, University of Modena and Reggio Emilia, via Campi 287, 41100 Modena, Italy

关键词: Mitochondrion;    Cardiolipin;    Mitochondrial membrane potential;    Apoptosis;    Flow cytometry;    CL;    cardiolipin;    NAO;    nonyl acridine orange;    ΔΨ;    mitochondrial membrane potential;    JC-1;    5;    5′;    6;    6′-tetrachloro-1;    1′;    3;    3′-tetraethylbenzimidazolcarbocyanine iodide;    Val;    valinomycin;   

DOI  :  10.1016/S0014-5793(00)01861-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a cytofluorimetric approach, we studied intramitochondrial cardiolipin (CL) distribution in HCW-2 cells, an apoptosis-resistant clone of human HL-60 cells. In HL-60, about 50% of total CL is distributed in the outer leaflet of mitochondrial inner membrane, while in HCW-2 a significantly higher amount of CL (about 65%) is in that site. In basal conditions, HSW-2 cells also show a reduced mitochondrial membrane potential even if they are able to proliferate as the parental line. Taking into account the complex functions that CL plays in the regulation of mitochondrial activity, it is likely that HCW-2 could produce ATP utilizing more glycolytic pathways rather than mitochondrial respiratory chain.

【 授权许可】

Unknown