Phospholipid asymmetry of the outer membrane of rat liver mitochondria

FEBS Letters

Nicolay, Klaas¹  van der Linden, Peter²  de Kruijff, Ben¹  Thijssen, José²  Hovius, Ruud¹ 

[1] Institute of Molecular Biology and Medical Biotechnology, Utrecht University, Padualaan 8, 3584 Utrecht, The Netherlands;Department Biochemistry of Membranes (Centre for Biomembranes and Lipid Enzymology), Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

关键词: Rat liver;    Mitochondrion;    Outer membrane;    Phospholipid asymmetry;    Cardiolipin;    Phospholipase A2;    Phosphatidyleholine transfer protein;   

DOI  :  10.1016/0014-5793(93)80922-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The phospholipid topology of the outer membrane of intact rat liver mitochondria and derived outer membrane vesicles was investigated by determining the accessible pool of the various phospholipid classes towards phospholipase A₂, a phosphatidylcholine-specific transfer protein and by chemical labeling using trinitrobenzene sulfonic acid. The outer membrane vesicles are sealed and have a right-side-out topology with a proposed localization of 55%, 77%, 100%, and at least 30% of the phosphatidylcholine, phosphatidylethanolamine, cardiolipin, and phosphatidylinositol plus phosphatidylserine in the outer leaflet, respectively. The outer membrane in intact mitochondria appears to have a similar phospholipid distribution.

【 授权许可】

Unknown