Organisation of mitochondria in living sensory neurons

FEBS Letters

Dedov, Vadim N¹  Roufogalis, Basil D¹ 

[1] Department of Pharmacy, University of Sydney, Sydney, NSW 2006, Australia

关键词: Sensory neuron;    Mitochondrion;    Fluorescent probe 5;    5′;    6;    6′-tetrachloro-1;    1′;    3;    3′-tetraethylbenzimidazolo carbocyanine iodide;    Mitochondrial membrane potential;    DRG;    dorsal root ganglia;    JC-1;    5;    5′;    6;    6′-tetrachloro-1;    1′;    3;    3′-tetraethylbenzimidazolo carbocyanine iodide;   

DOI  :  10.1016/S0014-5793(99)00951-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this work, we have examined the mitochondrial organisation in living cultured primary dorsal root ganglion (DRG) neurons. Confocal microscopy and the mitochondrial potential-sensitive fluorescent dye 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolo carbocyanine iodide (JC-1) were used to visualise intracellular structures with a high and low membrane potential. Three-dimensional reconstruction revealed a mitochondrial organisation featuring separate highly polarised mitochondria, clusters of mitochondria located mainly at the base of neurite hillocks and filamentous mitochondrial structures. Filamentous mitochondria were distributed along the cell body, especially between neurites. A functional integration between mitochondrial structures is proposed.

【 授权许可】

Unknown