Production of polyomavirus structural protein VP1 in yeast cells and its interaction with cell structures

FEBS Letters

Štokrová, J²  Palková, Z¹  Forstová, J¹  Liebl, D¹  Adamec, T¹ 

[1] Department of Genetics and Microbiology, Charles University, Viničná 5, 12844 Prague 2, Czech Republic;Institute of Molecular Genetics, Flemingovo n. 2, 16000 Prague 6, Czech Republic

关键词: Polyomavirus VP1;    Expression in Saccharomyces cerevisiae;    Tubulin interaction;    Mitotic spindle;   

DOI  :  10.1016/S0014-5793(00)01787-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene for mouse polyomavirus major structural protein VP1 was expressed in Saccharomyces cerevisiae from the inducible GAL7 promoter. VP1 pseudocapsids were purified from cell lysates. Their subpopulation contained fragments of host DNA, which, in contrast to those of VP1 pseudocapsids produced in insect cells, did not assemble with cellular histones into pseudonucleocores. VP1 pseudocapsids accumulated in the yeast cell nuclei. A strong interaction of VP1 with tubulin fibres of the mitotic spindle was observed. The fibres of spindles were larger in diameter, apparently due to tight VP1 binding. Substantial growth inhibition of yeast cells producing VP1 was observed.

【 授权许可】

Unknown