Mouse polyomavirus large T antigen inhibits cell growth and alters cell and colony morphology in Saccharomyces cerevisiae

FEBS Letters

Adamec, T.¹  Forstová, J.¹  Palková, Z.¹ 

[1] Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, 128 44, Prague 2, Czech Republic

关键词: Polyomavirus large tumor antigen;    Expression in Saccharomyces cerevisiae;    Growth inhibition;    Cyclic adenosine monophosphate;   

DOI  :  10.1016/S0014-5793(03)01246-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene for mouse polyomavirus large tumor (LT) antigen, a potent oncoprotein, was expressed in Saccharomyces cerevisiae from the inducible GAL1 promoter. Substantial cell growth inhibition as well as colony and cell morphology changes dependent on cyclic adenosine monophosphate (cAMP) were observed. In contrast to cell and colony morphology alterations, the growth inhibition appeared to be transient, thus indicating the existence of an active adaptation of yeast cells to the LT antigen presence.

【 授权许可】

Unknown