Characterization of a C‐terminal‐type kinesin‐related protein from Dictyostelium discoideum

FEBS Letters

Suyama, Eigo¹  Sutoh, Kazuo¹  Adachi, Hiroyuki¹  Iwai, Sosuke¹ 

[1] Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan

关键词: Kinesin-related protein;    C-terminal-type kinesin;    Mitotic spindle;    Dictyostelium discoideum;    KRP;    kinesin-related protein;    GFP;    green fluorescent protein;   

DOI  :  10.1016/S0014-5793(00)01619-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have determined the full sequence of K2, a kinesin-related protein (KRP) in Dictyostelium discoideum. Sequence homology and domain organization placed K2 in the ncd/Kar3 subfamily of the C-terminal-type KRPs. Bacterially expressed, truncated K2 showed ATP-dependent binding to microtubules and microtubule-stimulated ATPase activity. K2-null cells grew and developed normally, suggesting overlapping functions of K2 with other microtubule motor(s). Overexpression of K2 caused partial mitotic arrest. Green fluorescent protein-tagged full-length K2 localized in the nucleus at the interphase and on the mitotic spindle during mitosis. These results suggest that K2 is a microtubule-dependent motor which may play some roles in mitotic spindles.

【 授权许可】

Unknown