Patch‐clamp study of liver nuclear ionic channels reconstituted into giant proteoliposomes

FEBS Letters

Rousseau, Eric²  Proteau, Sonia²  Escande, Denis¹  Payet, Marcel Daniel²  Guihard, Gilles¹ 

[1] INSERM U533, Hôtel-Dieu, 1 Place A. Ricordeau, 44000 Nantes, France;Department of Physiology and Biophysics, Faculty of Medicine, University of Sherbrooke, Sherbrooke, Que., Canada J1H 5N4

关键词: Nucleus;    Ion channel;    Ca2+ store;    Intracellular membrane;    NE;    nuclear envelope;    NIC;    nuclear ionic channel;    NPC;    nuclear pore complex;    SER;    sarco-endoplasmic reticulum;   

DOI  :  10.1016/S0014-5793(00)01752-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nuclear ionic channels (NICs) represent ubiquitous structures of living cells, although little is known about their functional properties and encoding genes. To characterize NICs, liver nuclear membrane vesicles were reconstituted into either planar lipid bilayers or proteoliposomes. Reconstitution of nuclear envelope (NE) vesicles into planar lipid bilayer proceeded with low efficiency. NE vesicle reconstitution into proteoliposomes led to NIC observations by the patch-clamp technique. Large conductance, voltage-gated, K⁺-permeant and Cl⁻-permeant NICs were characterized. An 80–105-pS K⁺-permeant NIC with conducting sub-state was also recorded. Our data establish that NICs can be characterized upon reconstitution into giant proteoliposomes and retain biophysical properties consistent with those described for native NICs.

【 授权许可】

Unknown