Dissociation of store release from transmembrane influx of calcium in human neutrophils

FEBS Letters

Hallett, M.B.¹  Davies, E.V.¹  Campbell, A.K.² 

[1] Molecular Signaling Group, Department of Surgery, University of Wales College of Medicine, Cardiff, CF4 4XN, UK;Molecular Signaling Group, Medical Biochemistry, University of Wales College of Medicine, Cardiff, CF4 4XN, UK

关键词: Neutrophil;    Ca2+ store;    Ca2+ cloud;    Ratio imaging;   

DOI  :  10.1016/0014-5793(92)81426-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Release of Ca²⁺ from intracellular stores was visualised in individual neutrophils in the presence of the Mn²⁺ or SKF 96365, Influx of Mn²⁺ quenched fura-2 close to the plasma membrane but did not quench fura-2 at the site of store release. The size and location of the ‘cloud’ of elevated Ca²⁺ was unaffected by the channel blocker SKF 96365. Furthermore, the size and location was unaffected by the presence of extracellular Ca²⁺. This dissociation of transmembrane influx from store release demonstrates that the entry of Ca²⁺ into the cytosol of neutrophils occurs directly into the cytosol and not via the store site.

【 授权许可】

Unknown