Synchronous free Ca2+ changes in individual neutrophils stimulated by leukotriene B4

FEBS Letters

Hallett, M.B.²  Davies, E.V.²  Campbell, A.K.¹ 

[1] Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff CF4 4XN, UK;Dept. Surgery, University of Wales College of Medicine, Heath Park, Cardiff CF4 4XN, UK

关键词: Neutrophil;    Ca2+ signalling;    Ratio imaging;    Leukotriene;   

DOI  :  10.1016/0014-5793(91)81121-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Calcium (Ca²⁺) signals were monitored in individual neutrophils using ratio imaging of fura-2. In contrast to N-formyl-L-leucyl-L-phenylaianine (f-met-leu-phe), which produced grossly asynchronous Ca²⁺ signals with delays in response (up to 60 s), leukotriene B₄ (LTB₄) provoked synchronous and immediate elevations in cytosolic free Ca²⁺. Some individual neutrophils which responded immediately to LTB₄, subsequently displayed delayed Ca²⁺ signals in response to f-met-leu-phe. A sub-population of neutrophils failed to respond to both LTB₄ and f-met-leu-phe. The asynchrony of the Ca²⁺ signalling to f-met-leu-phe is not, therefore, an obligatory property of signal transduction in neutraphils.

【 授权许可】

Unknown