The α2 and α5 integrin genes: identification of transcription factors that regulate promoter activity in epidermal keratinocytes

FEBS Letters

Watt, Fiona M¹  Jensen, Uffe Birk¹  Corbi, Angel L¹ 

[1] Keratinocyte Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK

关键词: Integrin promoter;    CCAAT/enhancer binding protein;    AP-1;    Differentiation;    Epidermis;    Keratinocyte;   

DOI  :  10.1016/S0014-5793(00)01591-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We analysed the activity of the proximal promoters of the α2 and α5 integrin genes in human keratinocytes. An AP-1 site, found in the α5 but not the α2 promoter, bound c-Jun/c-Fos dimers and contributed strongly to promoter activity. Both promoters had a CCAAT/enhancer binding protein (C/EBP) binding site: the α5 C/EBP element enhanced activity, while the α2 site was a negative regulatory element. C/EBP overexpression repressed the activity of both promoters, but the effect was independent of occupancy of the identified C/EBP binding sites, suggesting interactions with additional transcription factors. We propose that upregulation of C/EBPs contributes to the inhibition of integrin transcription during keratinocyte terminal differentiation, while AP-1 factors play a role in the selective induction of the α5 gene during wound healing.

【 授权许可】

Unknown