The skin‐type antifreeze protein gene intron of the winter flounder is a ubiquitous enhancer lacking a functional C/EBPα binding motif

FEBS Letters

Hew, Choy L²  Chan, Shing-Leng²  Miao, Ming³  Gong, Zhiyuan¹ 

[1] School of Biologial Sciences, National University of Singapore, Singapore, Singapore;Departments of Laboratory Medicine and Pathobiology, and Biochemistry, 100 College Street, Room 351, Toronto, Ont. M5G 1L5, Canada;Biochemistry Division, Research Institute, Hospital for Sick Children, University of Toronto, Toronto, Ont., Canada

关键词: Winter flounder;    Antifreeze protein;    Skin-type;    Liver-type;    CCAAT/enhancer binding protein;    AFP;    antifreeze binding protein;    C/EBP;    CCAAT/enhancer binding protein;    AP-1;    activator protein-1;    AEP;    antifreeze enhancer binding protein;    CAT;    chloramphenicol acetyltransferase;   

DOI  :  10.1016/S0014-5793(98)00327-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The winter flounder antifreeze protein (AFP) intron contains a liver-specific enhancer (Element B) which was shown earlier to bind CCAAT/enhancer binding protein (C/EBP)α. In contrast, as demonstrated in the present studies, the intron of the skin-type AFP gene acted as a ubiquitous enhancer and contained a TA insertion at similar region to Element B (Element S) which destroyed its interaction with C/EBPα. Furthermore, a TA insertion of Element B by site-directed mutagenesis decreased its liver enhancer activity. The presence or absence of C/EBPα binding motifs in Element B and Element S, respectively, may provide a mechanism for their differential expression.

【 授权许可】

Unknown