EST comparison indicates 38% of human mRNAs contain possible alternative splice forms

FEBS Letters

Bork, Peer¹  Lehmann, Gerrit¹  Brett, David¹  Haase, Sabine¹  Krueger, Steffen³  Reich, Jens¹  Delbrück, Sebastian²  Hanke, Jens¹ 

[1] Max-Delbrück-Centre for Molecular Medicine, Robert-Rössle-Strasse 10, Berlin-Buch 13125, Germany;GenProfile AG, Robert-Rössle-Str. 10, 13125 Berlin, Germany;AGOWA GmbH, Gleinicker Weg 185, D-12489 Berlin, Germany

关键词: Alternative splicing;    Disease gene;    Database;   

DOI  :  10.1016/S0014-5793(00)01581-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expressed sequence tag (EST) databases represent a large volume of information on expressed genes including tissue type, expression profile and exon structure. In this study we create an extensive data set of human alternative splicing. We report the analysis of 7867 non-redundant mRNAs, 3011 of which contained alternative splice forms (38% of all mRNAs analysed). From a total of 12 572 ESTs 4560 different possible alternative splice forms were detected. Interestingly, 70% of the alternative splice forms correspond to exon deletion events with only 30% exonic insertions. We experimentally verified 19 different splice forms from 16 genes in a total subset of 20 studied; all of the respective genes are of medical relevance.

【 授权许可】

Unknown