Tissue‐specific expression of splice variants of the mouse voltage‐gated calcium channel α2/δ subunit

FEBS Letters

Angelotti, Timothy¹  Hofmann, Franz¹ 

[1] Institut für Pharmakologie and Toxikologie der Technischen Universität München, Biedersteinerstr. 29, 80802 München, Germany

关键词: Calcium channel;    Tissue-specific expression;    Alternative splicing;    RNAse protection assay;   

DOI  :  10.1016/S0014-5793(96)01205-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Five different splice variants of mouse α2/δ subunit isoforms (α2/a-e), which arose from various combinations of three alternatively spliced regions, were cloned with a combination of cDNA library screening and RT-PCR. Expression patterns and relative abundance of the various isoforms in mouse tissues were determined with an RNAse protection assay. Skeletal muscle and brain expressed single isoforms, α2a and α2b, respectively; however, the cardiovascular system expressed all five isoforms. Heart expressed mainly isoforms α2c and α2d while, in contrast to other species, aorta expressed predominantly α2a, the ‘skeletal muscle’ isoform. Smooth muscle-containing tissues expressed α2d and α2e. Thus, α2/δ isoforms are restricted in their tissue expression, suggesting an important functional role for the differentially spliced variants.

【 授权许可】

Unknown