Alternative splicing of the rat Cav3.3 T‐type calcium channel gene produces variants with distinct functional properties

FEBS Letters

Murbartián, Janet²  Perez-Reyes, Edward²  Gomora, Juan Carlos³  Arias, Juan Manuel²  Lee, Jung-Ha¹ 

[1] Department of Life Science, Sogang University, Shinsu-dong, Mapo-gu, Seoul 121-742, South Korea;Department of Pharmacology, University of Virginia, P.O. Box 800735, 1300 Jefferson Park Avenue, Charlottesville, VA 22908-0735, USA;Departamento de Biofı́sica, Instituto de Fisiologı́a Celular, UNAM, México D.F. 04510, Mexico

关键词: Calcium channel;    T-type;    Gene;    Alternative splicing;    Electrophysiology;    Rat;    Brain;   

DOI  :  10.1016/S0014-5793(02)03341-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Molecular diversity in T-type Ca²⁺ channels is produced by expression of three genes, and alternative splicing of those genes. Prompted by differences noted between rat and human Ca_v3.3 sequences, we searched for splice variants. We cloned six variants, which are produced by splicing at exon 33 and exon 34. Expression of the variants differed between brain regions. The electrophysiological properties of the variants displayed similar voltage-dependent gating, but differed in their kinetic properties. The functional impact of splicing was inter-related, suggesting an interaction. We conclude that alternative splicing of the Ca_v3.3 gene produces channels with distinct properties.

【 授权许可】

Unknown