Upregulation of thioredoxin (TRX) expression in giant cell myocarditis in rats

FEBS Letters

Nakayama, Yukie²  Kishimoto, Chiharu²  Shioji, Keisuke²  Sasayama, Shigekake²  Yodoi, Junji¹  Nakamura, Hajime¹  Toyokuni, Shinya³ 

[1] Department of Biological Responses, Institute for Virus Research, Kyoto University, 54 Kawaracho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan;Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawaracho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan;Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, 54 Kawaracho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan

关键词: Myocarditis;    Thioredoxin;    8-Hydroxy-2′-deoxyguanosine;    Nuclear factor kappa-B;   

DOI  :  10.1016/S0014-5793(00)01446-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To examine the possible involvement of a redox regulating mechanism in the pathogenesis of immune-mediated myocarditis, myocarditis was induced by immunization of porcine cardiac myosin in rats and immunohistochemistry and Western blot for thioredoxin (TRX) were performed. Immunohistochemistry for 8-hydroxy-2′-deoxyguanosine (8-OHdG) and nuclear factor kappa-B (NF-κB) was also performed. TRX was upregulated in the acute stage, but not in the chronic stage, and the expression was correlated with the severity of the disease. Damaged myocytes were strongly immunostained for 8-OHdG and NF-κB. Thus, TRX may be specifically induced by acute inflammatory stimuli, and the development of acute immune-mediated myocarditis may be regulated by the cellular redox state via TRX.

【 授权许可】

Unknown