A cytochrome c–GFP fusion is not released from mitochondria into the cytoplasm upon expression of Bax in yeast cells

FEBS Letters

Roucou, Xavier¹  Nagley, Phillip¹  Prescott, Mark¹  Devenish, Rodney J¹ 

[1] Department of Biochemistry and Molecular Biology, Monash University, Clayton, Vic. 3800, Australia

关键词: Cytochrome c;    Green fluorescent protein;    Bax;    Yeast;    Cell death;    Mitochondrion;   

DOI  :  10.1016/S0014-5793(00)01404-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To study Bax-induced release of cytochrome c in vivo, we have expressed a cytochrome c–GFP (green fluorescent protein) fusion in Saccharomyces cerevisiae cells null for the expression of the endogenous cytochrome. We show here that cytochrome c–GFP is efficiently localised to mitochondria and able to function as an electron carrier between complexes III and IV of the respiratory chain. Strikingly, while natural cytochrome c is released into the cytoplasm upon expression of Bax, the cytochrome c–GFP fusion is not. Nevertheless, cells co-expressing Bax and the cytochrome c–GFP fusion die, indicating that mitochondrial release of cytochrome c is not essential for cell death to occur in yeast. The failure to release cytochrome c–GFP is presumed to arise from increased bulk due to the GFP moiety. We propose that in intact yeast cells, Bax-induced release of cytochrome c into the cytoplasm occurs through a selective pore and not as a consequence of the non-specific breakage of the mitochondrial outer membrane.

【 授权许可】

Unknown