In vitro glucuronidation of kaempferol and quercetin by human UGT‐1A9 microsomes

FEBS Letters

Oliveira, E.J¹  Watson, D.G¹ 

[1] Department of Pharmaceutical Sciences, University of Strathclyde, Strathclyde Institute of Biomedical Sciences, 27 Taylor Street, Glasgow G4 ONR, UK

关键词: Quercetin;    Kaempferol;    Glucuronidation;    Liquid chromatography–mass spectrometry;    UDP-glucuronosyltransferase 1A9;    Ginkgo biloba;   

DOI  :  10.1016/S0014-5793(00)01355-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Flavonoids are important polyphenolic substances with widespread occurrence in plants and therefore in the human diet. Although considerable work has been done on the pharmacology of flavonoids, the understanding of their metabolism is still incomplete. In this work, the in vitro glucuronidation of the common dietary flavonoids quercetin and kaempferol by human UDP-glucuronosyltransferase microsomes (UGT-1A9) was investigated using HPLC and LC–MS. The two flavonoids were extensively metabolised by this enzyme with four monoglucuronides of quercetin and two of kaempferol being detected after incubation. The presence of a quercetin monoglucuronide in the urine of a volunteer after consumption of Ginkgo biloba tablets was demonstrated.

【 授权许可】

Unknown