Antibacterial effect of crude extract and metabolites of Phytolacca americana on pathogens responsible for periodontal inflammatory diseases and dental caries

BMC Complementary and Alternative Medicine

Research Article

Yangseon Kim¹  Hyeon-Jeong Kim¹  Jayanta Kumar Patra²  Kwang-Hyun Baek²  Eun Sil Kim³  Kyounghee Oh³ 

[1] Ensoltek Co., Ltd., Techno 10-ro 51, 305-510, Yuseong-gu, Daejeon, Republic of Korea;School of Biotechnology, Yeungnam University, 712-749, Gyeongsan, Gyeongbuk, Republic of Korea;Wildlife Genetic Resources Center, National Institute of Biological Resources, 404-708, Incheon, Republic of Korea;

关键词: Kaempferol;    Phytolacca americana;    Porphyromonas gingivalis;    Quercetin;    Streptococcus mutans;   

DOI  :  10.1186/1472-6882-14-343

 received in 2014-06-18, accepted in 2014-09-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe oral cavity is the store house of different species of microorganisms that are continuously engaged in causing diseases in the mouth. The present study was conducted to evaluate the antibacterial potential of crude extracts of the aerial parts of Phytolacca americana and its natural compounds against two oral pathogens, Porphyromonas gingivalis and Streptococcus mutans, which are primarily responsible for periodontal inflammatory diseases and dental caries, as well as a nonpathogenic Escherichia coli.MethodsCrude extract and fractions from the aerial parts of P. americana (0.008–1.8 mg/mL) were evaluated for their potential antibacterial activity against two oral disease causing microorganisms by micro-assays. The standard natural compounds present in P. americana, kaempferol, quercetin, quercetin 3-glucoside, isoqueritrin and ferulic acid, were also tested for their antibacterial activity against the pathogens at 1–8 μg/mL.ResultsThe crude extract was highly active against P. gingivalis (100% growth inhibition) and moderately active against S. mutans (44% growth inhibition) at 1.8 mg/mL. The chloroform and hexane fraction controlled the growth of P. gingivalis with 91% and 92% growth inhibition at a concentration of 0.2 mg/mL, respectively. Kaempferol exerted antibacterial activity against both the pathogens, whereas quercetin showed potent growth inhibition activity against only S. mutans in a concentration dependent manner.ConclusionThe crude extract, chloroform fraction, and hexane fraction of P. americana possesses active natural compounds that can inhibit the growth of oral disease causing bacteria. Thus, these extracts have the potential for use in the preparation of toothpaste and other drugs related to various oral diseases.

【 授权许可】

Unknown   
© Patra et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.