FEBS Letters | |
Slow formation of [3Fe–4S]1+ clusters in mutant forms of Desulfovibrio africanus ferredoxin III | |
Davy, Sharon L.2  Busch, Johanneke L.H.2  Hannan, Jonathan P.2  James, Richard1  Thomson, Andrew J.2  Moore, Geoffrey R.2  | |
[1] School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK;School of Chemical Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK | |
关键词: Di-cluster ferredoxin; Cluster formation; 1H NMR spectroscopy; | |
DOI : 10.1016/S0014-5793(00)01210-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Desulfovibrio africanus ferredoxin III (Da FdIII) readily interconverts between a 7Fe and an 8Fe form with Asp-14 believed to provide a cluster ligand in the latter form. To investigate the factors important for cluster interconversion in Fe/S cluster-containing proteins we have studied two variants of Da FdIII produced by site-directed mutagenesis, Asp14Glu and Asp14His, with cluster incorporation performed in vitro. Characterisation of these proteins by UV/visible, EPR and 1H NMR spectroscopies revealed that the formation of the stable 7Fe form of these proteins takes some time to occur. Evidence is presented which indicates the [4Fe–4S]2+ cluster is incorporated prior to the [3Fe–4S]1+ cluster.
【 授权许可】
Unknown
【 预 览 】
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RO201912020309053ZK.pdf | 110KB | download |