Slow formation of [3Fe–4S]1+ clusters in mutant forms of Desulfovibrio africanus ferredoxin III

FEBS Letters

Davy, Sharon L.²  Busch, Johanneke L.H.²  Hannan, Jonathan P.²  James, Richard¹  Thomson, Andrew J.²  Moore, Geoffrey R.² 

[1] School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK;School of Chemical Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK

关键词: Di-cluster ferredoxin;    Cluster formation;    1H NMR spectroscopy;   

DOI  :  10.1016/S0014-5793(00)01210-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Desulfovibrio africanus ferredoxin III (Da FdIII) readily interconverts between a 7Fe and an 8Fe form with Asp-14 believed to provide a cluster ligand in the latter form. To investigate the factors important for cluster interconversion in Fe/S cluster-containing proteins we have studied two variants of Da FdIII produced by site-directed mutagenesis, Asp14Glu and Asp14His, with cluster incorporation performed in vitro. Characterisation of these proteins by UV/visible, EPR and ¹H NMR spectroscopies revealed that the formation of the stable 7Fe form of these proteins takes some time to occur. Evidence is presented which indicates the [4Fe–4S]²⁺ cluster is incorporated prior to the [3Fe–4S]¹⁺ cluster.

【 授权许可】

Unknown