First evidence and characterization of an uncoupling protein in fungi kingdom: CpUCP of Candida parapsilosis

FEBS Letters

Jarmuszkiewicz, Wiesława¹  Schreiber, Angelica Z.²  Sluse, Francis E.³  Vercesi, Anibal E.⁴  Milani, Graziela⁴  Fortes, Fabiane⁴ 

[1] Department of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701 Poznan, Poland;Laboratorio de Microbiologia, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, 13083-970 Campinas, SP, Brazil;Laboratory of Bioenergetics, Centre of Oxygen, Research and Development, Institute of Chemistry B6, University of Liège, Sart Tilman, B-4000 Liège, Belgium;Departamento de Patologia Clı́nica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, CP 6111, 13083-970 Campinas, SP, Brazil

关键词: Yeast mitochondrion;    Uncoupling protein;    Candida parapsilosis;    AOX;    alternative oxidase;    BHAM;    benzohydroxamate;    BSA;    bovine serum albumin;    CAT;    carboxyatractyloside;    CpUCP;    uncoupling protein of C. parapsilosis;    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;    FFA;    free fatty acid;    LA;    linoleic acid;    UCP;    uncoupling protein;    ΔΨ;    mitochondrial transmembrane electrical potential;   

DOI  :  10.1016/S0014-5793(00)01138-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An uncoupling protein (UCP) was identified in mitochondria from Candida parapsilosis (CpUCP), a non-fermentative parasitic yeast. CpUCP was immunodetected using polyclonal antibodies raised against plant UCP. Activity of CpUCP, investigated in mitochondria depleted of free fatty acids, was stimulated by linoleic acid (LA) and inhibited by GTP. Activity of CpUCP enhanced state 4 respiration by decreasing ΔΨ and lowered the ADP/O ratio. Thus, it was able to divert energy from oxidative phosphorylation. The voltage dependence of electron flux indicated that LA had a pure protonophoretic effect. The discovery of CpUCP proves that UCP-like proteins occur in the four eukaryotic kingdoms: animals, plants, fungi and protists.

【 授权许可】

Unknown