Metabolite transport in isolated yeast mitochondria: fumarate/malate and succinate/malate antiports

FEBS Letters

Passarella, Salvatore¹  Pallotta, Maria Luigia¹  Fratianni, Alessandra¹ 

[1] Dipartimento di Scienze Animali, Vegetali e dell'Ambiente, via F. De Sanctis, Università del Molise, 86100 Campobasso, Italy

关键词: Yeast mitochondrion;    Carrier;    Fumarate;    ADP;    ATP;    Transport;    Ap5A;    P1P5-diadenosine (-5′-pentaphosphate);    SCM;    Saccharomyces cerevisiae mitochondria;   

DOI  :  10.1016/S0014-5793(99)01535-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study, we investigated the metabolite permeability of isolated coupled Saccharomyces cerevisiae mitochondria. The occurrence of a fumarate/malate antiporter activity was shown. The activity differs from that of the dicarboxylate carrier (which catalyses the succinate/malate antiport) in (a) kinetics (K _m and V _max values are about 27 μM and 22 nmol min⁻¹ mg protein⁻¹ and 70 μM and 4 nmol min⁻¹ mg protein⁻¹, respectively), (b) sensitivity to inhibitors, (c) K _i for the competitive inhibitor phenylsuccinate and (d) pH profiles.

【 授权许可】

Unknown