期刊论文详细信息
FEBS Letters
pH control of the plant outwardly‐rectifying potassium channel SKOR
Sentenac, Hervé1  Pilot, Guillaume1  Gaymard, Frédéric1  Lacombe, Benoît1  Thibaud, Jean-Baptiste1 
[1] Biochimie et Physiologie Moléculaire des Plantes, UMR 5004 Agro-M/CNRS/INRA/UM2, Place Viala, 34060 Montpellier Cedex 1, France
关键词: K+ channel;    pH;    Xenopus oocyte;    Patch clamp;    Arabidopsis thaliana;   
DOI  :  10.1016/S0014-5793(00)01093-0
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
PDF
【 摘 要 】

SKOR, an Arabidopsis depolarisation-activated K+-selective channel, was expressed in Xenopus oocytes, and external and internal pH effects were analysed. Internal pH was manipulated by injections of alkaline or acidic solutions or by acid load from acetate-containing medium. An internal pH decrease from 7.4 to 7.2 induced a strong (ca. 80%) voltage-independent decrease of the macroscopic SKOR current, the macroscopic gating parameters and the single channel conductance remained unchanged. An external acidification from 7.4 to 6.4 had similar effects. It is proposed that pH changes regulate the number of channels available for activation. Sensitivity of SKOR activity to pH in the physiological range suggests that internal and external pH play a role in the regulation of K+ secretion into the xylem sap.

【 授权许可】

Unknown   

【 预 览 】
附件列表
Files Size Format View
RO201912020308933ZK.pdf 122KB PDF download
  文献评价指标  
  下载次数:4次 浏览次数:5次