FEBS Letters | |
Quinine inhibits Ca2+‐independent K+ channels whereas tetraethylammonium inhibits Ca2+‐activated K+ channels in insulin‐secreting cells | |
Ullrich, Susanne1  Wollheim, Claes B.1  Petersen, Ole H.1  Dunne, Mark J.1  Findlay, Ian1  | |
[1] MRC Secretory Control Research Group, The Physiological laboratory, University of Liverpool, PO Box 147, Brownlow Hill, Liverpool L69 3BX, England | |
关键词: RINm5F cell; Patch clamp; K+ channel; Tetraethylammonium; Quinine; | |
DOI : 10.1016/0014-5793(85)80729-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The effects of quinine and tetraethylammonium (TEA) on single-channel K+ currents recorded from excised membrane patches of the insulin-secreting cell line RINm5F were investigated. When 100 μM quinine was applied to the external membrane surface K+ current flow through inward rectifier channels was abolished, while a separate voltage-activated high-conductance K+ channel was not significantly affected. On the other hand, 2 mM TEA abolished current flow through voltage-activated high-conductance K+ channels without influencing the inward rectifier K+ channel. Quinine is therefore not a specific inhibitor of Ca2+-activated K+ channels, but instead a good blocker of the Ca2+-independent K+ inward rectifier channel whereas TEA specifically inhibits the high-conductance voltage-activated K+ channel which is also Ca2+-activated.
【 授权许可】
Unknown
【 预 览 】
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