FEBS Letters | |
Protein phosphorylation is required for diazoxide to open ATP‐sensitive potassium channels in insulin (RINm5F) secreting cells | |
Dunne, M.J.1  | |
[1] MRC Secretory Control Research Group, The Department of Physiology, The University of Liverpool, Liverpool L69 3BX, England | |
关键词: Patch clamp; K+ channel; Diazoxide; ATP; (RINm5F cell); ATP; adenosine 5′-triphosphate; AMP-PNP; adenylyl-imidodiphosphate; AMP-PCP; methyleneadenosine 5′-triphosphate; ATPγS; adenosine 5′-O-(3-thiotriphosphate); | |
DOI : 10.1016/0014-5793(89)80734-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The patch-clamp open-cell recording configuration has been used to investigate the effects of non-hydrolyzable analogues of ATP on the diazoxide-activation of KATP channels in the insulin-secreting cell line RINm5F. K+ channels inhibited by 0.1, 0.5 and 1.0 mM ATP were consistently activated by 200 μM diazoxide. During sustained activation of channels, exchange of ATP for either AMP-PNP, AMP-PCP or ATPγS abolished the effects of diazoxide. If diazoxide was added to the membrane in the continued presence of AMP-PNP, AMP-PCP or ATPγS either no effects were observed or alternatively a small transient activation of channels occurred. This study suggests that protein phosphorylation is necessary for diazoxide to activate ATP-sensitive potassium channels in insulin-secreting cells.
【 授权许可】
Unknown
【 预 览 】
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