Characterization of a testicular 17α,20β‐dihydroxy‐4‐pregnen‐3‐one (a spermiation‐inducing steroid in fish) receptor from a teleost, Japanese eel (Anguilla japonica)

FEBS Letters

Kajiura-Kobayashi, Hiroko¹  Kobayashi, Tohru¹  Nagahama, Yoshitaka¹  Suzuki, Kunimasa¹  Ikeuchi, Toshitaka¹  Yoshikuni, Michiyasu¹  Yamauchi, Kohei²  Todo, Takashi¹ 

[1] Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan;Department of Biology, Faculty of Fisheries, Hokkaido University, Hakodate 041-8611, Japan

关键词: Nuclear receptor;    Progestin;    Spermiation;    Sperm maturation;    Japanese eel;   

DOI  :  10.1016/S0014-5793(99)01714-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding a nuclear 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DP, spermiation-inducing hormone in fish) receptor (DPR) was, for the first time, isolated from an eel testis cDNA library. The amino acid sequence of DPR shows high homology with those of human and chicken progesterone receptors. The affinity of the bacterial recombinant DPR ligand binding domain protein for 17α,20β-DP is higher than that of progesterone. In transfection experiments using COS7 cells, the DPR showed progestin-dependent activation of transcription. 17α,20β-DP was the most effective activator of transcription. These results indicate that the cDNA encodes a functional eel DPR, and show that 17α,20β-DP has a nuclear receptor-mediated action in eel testes.

【 授权许可】

Unknown