A novel progestogen receptor subtype in the Japanese eel, Anguilla japonica 1

FEBS Letters

Kobayashi, Tohru¹  Nagahama, Yoshitaka¹  Ikeuchi, Toshitaka¹  Todo, Takashi¹ 

[1] Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan

关键词: Nuclear receptor;    Progesterone receptor β;    Progestogen;    Spermiation;    Sperm maturation;    Japanese eel;   

DOI  :  10.1016/S0014-5793(01)03220-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding a second type of a progestogen receptor (ePR2) was isolated from the same library as we had previously cloned a functional PR (ePR1) in eel testis. The amino acid sequence of the ePR2 shows low homology with ePR1 (34%), but both PRs showed progestogen-dependent transactivation in transfection experiment. Tissue distribution of ePR2 mRNA was clearly different from that of ePR1. Protein interaction between two PRs was demonstrated in vitro by a glutathione S-transferase pull-down assay. These results indicate that ePR2 is also a functional PR. This is the first isolation of two different functional PR molecules from a vertebrate.

【 授权许可】

Unknown