In-depth Proteomic mapping of mouse (Mus musculus) epididymal constructive basis for sperm maturation

Proteome Science

Research Article

Fu-Jun Liu¹  Xiao-Fang Shen¹  Xin Liu¹  Yan-Wei Wang¹  Shao-Hua Jin² 

[1] Central Laboratory, Yantai Yu Huang Ding Hospital/Qingdao University, 264000,, Yantai, Shandong, People’s Republic of China;Clinical Laboratory, Yantai Yu Huang Ding Hospital/Qingdao University, 264000,, Yantai, Shandong, People’s Republic of China;

关键词: Sperm maturation;    Structural proteins;    Mouse epididymis;    Bioinformatics;    Proteomics;   

DOI  :  10.1186/s12953-015-0076-3

 received in 2015-04-07, accepted in 2015-07-20,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe mouse epididymis performs an essential role in sperm maturation, but global protein expression data in mouse epididymis are still lacking. Here, we reported the first in-depth gel-based profiling of mouse epididymis proteome and established a 2-DE map.ResultsA total of 832 protein spots were detected in the reproducible gels, and 625 spots corresponding to 355 unique protein entries have been successfully identified by MALDI-TOF-MS. The confidence of proteome data was validated by Western blot. Functional annotations showed that these proteins were mainly related to general metabolism, antioxidant and structural molecule activity. Immunohistochemistry disclosed two structural proteins (myosin regulatory light polypeptide 9 and alpha-2 type I collagen) continuously expressed in the myoid cell since postpartum.ConclusionThis study provides a first-draft reference map of the mouse epididymis proteome, which will greatly expand the knowledge of the epididymal structural basis and contribute to the better understanding of those proteins in the process of mouse epididymal sperm maturation.

【 授权许可】

CC BY   
© Liu et al. 2015