Non‐genomic effects of progesterone on the signaling function of G protein‐coupled receptors

FEBS Letters

Burger, Katja¹  Fahrenholz, Falk¹  Gimpl, Gerald¹ 

[1] Johannes Gutenberg-Universität Mainz, Institut für Biochemie, Becherweg 30, 55099 Mainz, Germany

关键词: Progesterone;    Non-genomic effect;    G protein-coupled receptor;    Calcium signal;    Anisotropy;    AVP;    (Arg8)-vasopressin;    [Ca2+]i;    intracellular free calcium ion concentration;    CCK-8;    cholecystokinin octapeptide (26–33) sulfated;    CHO;    Chinese hamster ovary;    DPH;    1;    6-diphenyl-1;    3;    5-hexatriene;    GPCR;    G protein-coupled receptor;    HEK;    human embryonic kidney;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(99)01668-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Progesterone at concentrations between 10 μM and 200 μM affected the calcium signaling evoked by ligand stimulation of G protein-coupled receptors expressed in several cell lines. At 160 μM progesterone the signaling of all receptors was completely abolished. The effect of progesterone was fast, reversible and was not prevented by cycloheximide indicating its non-genomic nature. Overall, the action of progesterone was more cell type-specific than receptor-specific. Our results are in contrast to a recent report [Grazzini, E., Guillon, G., Mouillac, B. and Zingg, H.H. (1998) Nature 392, 509–512] in which a direct high-affinity interaction between the oxytocin receptor and progesterone was suggested.

【 授权许可】

Unknown