Single cell observation of ligand‐induced desensitization of B‐cell membrane immunoglobulin‐mediated calcium signals

FEBS Letters

Mizuguchi, Junichiro¹  Nakanishi, Mamoru²  Noji, Masahide²  Yamada, Hiroshi² 

[1] Department of Immunology and Serology, Tokyo Medical College, 6-1-1 Shinjuku-ku, Tokyo 160, Japan;Faculty of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, Nagoya 467, Japan

关键词: Calcium signal;    Fluorescence imaging;    B cell;    Membrane immunoglobulin;    Protein kinase C;   

DOI  :  10.1016/0014-5793(92)81329-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a digital imaging fluorescence microscope we have observed the membrane immunoglobulin (mIg)-induced desensitization of calcium signals in individual BAL17 B lymphoma cells which express two kinds of antigen receptors, mIgM and mIgD. The mIgD-mediated desensitization was partly abrogated by pretreating the cells with phorbol 12-myristate 13-acetate (PMA) for 24 h, however, the mIgM-mediated one was not affected by the pretreatment. This supports the idea that at least two mechanisms are operative for mIg-induced desensitization in B cells.

【 授权许可】

Unknown