Participation of tyrosine kinase in capping, internalization, and antigen presentation through membrane immunoglobulin in BAL17 B lymphoma cells

FEBS Letters

Mizuguchi, Junichiro¹  Gyotoku, Yuichi⁴  Arimitsu, Yoshiko²  Kakiuchi, Terutaka³  Shimo, Kuniaki¹ 

[1] Department of Immunology and Serology, Tokyo Medical College, 6-1-1 Shinjuku-ku, Tokyo 160, Japan;Department of Bacteriology, National Institute of Health, 1-23-1 Toyama-cho, Shinjuku-ku, Tokyo 162, Japan;Department of Immunology, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, Tokyo 143, Japan;Life Science Laboratories,Central Research Laboratories, Ajinomoto Co. Inc., 214 Maeda-cho, Takatsu-ku, Yokohama 244, Japan

关键词: B cell;    Capping;    Membrane immunoglobulin;    Antigen presentation;    Tyrosine kinase;    Internalization;    Ig;    immunoglobulin;    PLC;    phospholipase C;    FITC;    fluorescein isothiocyanate;    MHC;    major histocompatibility complex;   

DOI  :  10.1016/0014-5793(93)81473-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

BAL17 cells pulsed with goat anti-IgM or anti-IgD as antigens stimulated a goat IgG specific T cell clone in terms of inositol phosphate production. The antigen-presenting capacity of BAL17 cells was inhibited by pretreatment with the tyrosine kinase inhibitors herbimycin A or genistein. Furthermore, ligand-induced capping and endocytosis of membrane immunoglobulin, monitored at the single cell level, was also blocked by herbimycin A. These results indicate that tyrosine phosphorylation plays an important role in receptor-mediated antigen presentation by B cells.

【 授权许可】

Unknown