Spectrin and protein 4.1 as an actin filament capping complex

FEBS Letters

Ohanian, V.¹  Gratzer, W.B.¹  Pinder, Jennifer C.¹ 

[1] Medical Research Council Cell Biophysics Unit, King's College, Drury Lane, London WC2B 5RL, England

关键词: Erythrocyte;    Cytoskeleton;    Spectrin;    Protein 4.1;    Actin;    Capping;   

DOI  :  10.1016/0014-5793(84)80310-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spectrin and protein 4.1, when added to G- or F-actin, cause the formation of short filaments, as judged by the appearance of powerful nucleating activity for G-actin polymerisation. F-Actin filaments are rapidly fragmented under physiological solvent conditions. The effect of cytochalasin E on the polymerisation reaction and the extent of reduction in the critical monomer concentration of actin when spectrin and 4.1 are added suggest that these proteins form a capping system for the more slowly growing, or ‘pointed’ ends of actin filaments. The interaction is not affected by calcium or by 4.9, the remaining constituent of the purified red cell membrane cytoskeleton.

【 授权许可】

Unknown