Selective detection of rapid motions in spectrin by NMR

FEBS Letters

Johnson, M.E.²  Hjelm, R.P.²  Lu, H.-Z.¹  Fung, L.W.-M.¹ 

[1] Department of Chemistry, Loyola University of Chicago, Chicago, IL 60626, USA;Department of Medicinal Chemistry & Pharmacognosy, University of Illinois at Chicago, Chicago, IL 60680, USA

关键词: Erythrocyte membrane Membrane skeleton Spectrin-actin complex Spectrin Internal motion NMR;   

DOI  :  10.1016/0014-5793(86)80333-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human erythrocyte spectrin molecules exhibit relatively sharp (30–50 Hz) proton NMR signals in the aliphatic region. A standard solvent presaturation pulse sequence that also partially suppresses the broad envelope from protons with rigid structures in spectrin and selectively enhances the sharp resonances has been used to characterize the behavior of these resonances. The overall resonance pattern strongly resembles that of the denatured spectrin. The observed spectra are also quite similar to the line-broadened spectrum from a mixture of amino acids that corresponds to the composition of the spectrin molecule. These data indicate the existence of regions exhibiting rapid internal motions within the intact spectrin molecule, and suggest that the amino acid composition of the residues giving rise to the sharp resonances is quite similar to that of the full spectrin molecule.

【 授权许可】

Unknown