A single cell observation of staurosporine effect on the Ca2+ signals in rat basophilic leukemia cells

FEBS Letters

Nakanishi, Mamoru²  Terao, Tadao³  Ikebuchi, Hideharu³  Miyagawa, Takehiko¹  Arata, Yoji¹  Teshima, Reiko³ 

[1] Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Faculty of Pharmaceutical Sciences, Nagoya City University, Tanabe-Dori, Mizuho-ku, Nagova 467, Japan;National Institute of Hygienic Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158, Japan

关键词: RBL-2H3;    Calcium signal;    Staurosporine;    Microscopic image analysis;   

DOI  :  10.1016/0014-5793(90)81247-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A digital imaging fluorescence microscope was used to study the effect of a protein kinase inhibitor staurosporine on the antigen-dependent calcium signals in an individual rat basophilic leukemia cell (RBL-2H3). Although dose dependency of staurosporine was different from one cell to another, staurosporine inhibited, at low concentration, the calcium influx from the external medium into RBL-2H3 cells. At high concentration, however, it inhibited both the removal of calcium ion from internal stores and the calcium influx from the external medium. These results indicated that staurosporine is necessary for the inhibition of the calcium influx from the external medium and that a protein kinase (possibly protein kinase C) is involved in the calcium influx from the external medium into the cytoplasm.

【 授权许可】

Unknown