Antigen receptor‐mediated calcium signals in B cells as revealed by confocal fluorescence microscopy

FEBS Letters

Mizuguchi, Junichiro¹  Nakanishi, Mamoru²  Yamada, Hiroshi² 

[1] Department of Applied Immunology, National Institute of Health, Kamiosaki, Sinagawa-ku, Tokyo 141, Japan;Faculty of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, Nagoya 467, Japan

关键词: Confocal fluorescence microscope;    Calcium signal;    Nucleus;    B cell;    Antigen receptor;   

DOI  :  10.1016/0014-5793(91)80696-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A confocal fluorescence microscope was used to study the antigen receptor-mediated calcium signals in B cells. Anti-IgD binding to B lymphoma cells (BAL17) increased the intracellular calcium concentration with short lag times. Confocal fluorescence images of the fluo-3-loaded BAL17 cells showed that the intracellular calcium ion concentrations increased non-homogeneously, suggesting that the calcium signals transferred not only to the cytoplasm but also to the nucleus.

【 授权许可】

Unknown