BMC Immunology | |
Morphological change of CD4 + T cell during contact with DC modulates T-cell activation by accumulation of F-actin in the immunology synapse | |
Yiwei Chu1  Xunbin Wei2  Yijie Zheng1  Zhichao Fan2  Yuting Deng1  Yuanzhen Suo2  Wei Lin1  | |
[1] Biotherapy Research Centre, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China;Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China | |
关键词: Calcium signal; Immunological synapse; F-actin; T-cell activation; | |
Others : 1222754 DOI : 10.1186/s12865-015-0108-x |
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received in 2015-04-10, accepted in 2015-07-09, 发布年份 2015 | |
【 摘 要 】
Background
The changes in T-cell morphology during immunological synapse (IS) formation are essential for T-cell activation. Previous researches have shown that T cell changed from spherical to elongated and/or flattened during in contact with B cell. As most powerful antigen presenting cell, dendritic cell (DC) has a strong ability to activate T cells. However, the morphological change of T cell which contacts DC and the relationship between morphological change and T-cell activation are not very clear. Thus, we studied the morphological change of CD4 +T cell during contact with DC.
Results
Using live-cell imaging, we discovered diversity in the T-cell morphological changes during contact with DCs. The elongation-flattening of CD4 +T cells correlated with a low-level Ca 2+response and a loss of T-cell receptor (TCR) signalling molecules in the IS, including zeta-chain associated protein kinase 70 (ZAP-70), phospholipase C-γ (PLC-γ) and protein kinase C-θ (PKC-θ), whereas rounding-flattening correlated with sufficient CD4 +T-cell activation. Different morphological changes were correlated with the different amount of accumulated filamentous actin (F-actin) in the IS. Disruption of F-actin by cytochalasin D impaired the morphological change and the localisation of calcium microdomains in the IS and decreased the calcium response in CD4 +T cells.
Conclusion
Our study discovered the diversity in morphological change of T cells during contacted with DCs. During this process, the different morphological changes of T cells modulate T-cell activation by the different amount of F-actin accumulation in the IS, which controls the distribution of calcium microdomains to affect T-cell activation.
【 授权许可】
2015 Lin et al.
【 预 览 】
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