The E‐box DNA binding protein Sgc1p suppresses the gcr2 mutation, which is involved in transcriptional activation of glycolytic genes in Saccharomyces cerevisiae

FEBS Letters

Sugioka, Shigemi¹  Uemura, Hiroshi¹  Baker, Henry V.²  Sato, Takashi¹  Jigami, Yoshifumi¹  Lopez, M.Cecilia² 

[1] Department of Molecular Biology, National Institute of Bioscience and Human-Technology, Tsukuba Research Center (MITI), Higashi 1-1, Tsukuba, Ibaraki 305-8566, Japan;Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, FL 32610-0266, USA

关键词: Sgc1p;    Gcr2p;    Transcriptional activation;    Glycolysis;    Yeast;    Saccharomyces cerevisiae;    bHLH;    basic/helix-loop-helix;    IPTG;    isopropyl-β-D-thiogalactopyranoside;    Eno;    enolase;    Gpm;    phosphoglycerate mutase;    Pyk;    pyruvate kinase;    Zwf;    glucose-6-P dehydrogenase;   

DOI  :  10.1016/S0014-5793(99)01654-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glycolytic gene expression is mediated by the Gcr1p-Gcr2p transcriptional activation complex. A screen for multicopy suppressors of gcr2 yielded SGC1. SGC1's suppression activity was specific to gcr2, it did not extend to gcr1. Disruption of SGC1 moderately affected glycolytic enzyme activities, although no growth defect was evident. Sgc1p exhibits a bHLH motif which is characteristic of E-box DNA-binding proteins. DNA footprinting experiments demonstrated Sgc1p's ability to bind at an E-box. However, its binding specificity was less than 10-fold, which is also characteristic of E-box binding proteins. LexA fusion experiments demonstrated that Sgc1p has weak intrinsic activating activity independent of GCR1 and GCR2. We propose that Sgc1p binds at E-boxes of glycolytic genes and contributes to their activation.

【 授权许可】

Unknown